A549 cells or NCI-1650 cells were seeded into 6-wellplates and cultured until they reached 100% confluency. Themonolayer of cells was scratched using a 200-µl sterile pipettetip, and the culture was continued for another 48 h in serum-freemedium. Wound closure was imaged at 0 and 48 h using a lightmicroscope (Nicon Eclipase E600; Nikon Corporation). The width ofthe wound was measured using ImageJ (version 1.48u; NationalInstitutes of Health), and the relative wound width was calculatedto indicate cell migration.
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